GB 1903.43-2020 English PDF

Delivery: 2 working-hours manually (Sales@ChineseStandard.net)
Need delivered in 3-second? USA-Site: GB 1903.43-2020
Get Quotation: Click GB 1903.43-2020 (Self-service in 1-minute)
Historical versions (Master-website): GB 1903.43-2020
Preview True-PDF (Reload/Scroll-down if blank)

GB 1903.43-2020: National food safety standard - Food nutritional fortification substance - Cyanocobalamin cobione
GB 1903.43-2020
GB
NATIONAL STANDARD OF THE
PEOPLE?€?S REPUBLIC OF CHINA
National Food Safety Standard - Food Nutritional
Fortification Substance ?€? Cyanocobalamin cobione
ISSUED ON: SEPTEMBER 11, 2020
IMPLEMENTED ON: MARCH 11, 2021
Issued by: National Health Commission of the People?€?s Republic of China;
State Administration for Market Regulation.
Table of Contents
1 Scope ... 3
2 Molecular Formula, Structural Formula and Relative Molecular Mass ... 3
3 Technical Requirements ... 4
Appendix A Test Methods ... 5
Appendix B Infrared Spectrum of Cyanocobalamin Standard Product ... 14
Appendix C Acetone Gas Chromatogram ... 15
National Food Safety Standard - Food Nutritional
Fortification Substance - Cyanocobalamin Cobione
1 Scope
This Standard is applicable to cyanocobalamin, a food nutritional fortification
substance obtained through transformation and crystallization of fermentation broth
containing cobalamin produced by microbial fermentation.
2 Molecular Formula, Structural Formula and Relative
Molecular Mass
2.1 Molecular formula
C63H88CoN14O14P
2.2 Structural formula
2.3 Relative molecular mass
Cyanocobalamin: 1355.38 (according to 2018 international relative atomic mass)
Appendix A
Test Methods
A.1 Warning
Some reagents used in the test methods of this Standard are toxic or corrosive, and
appropriate safety and protective measures shall be taken during operation.
A.2 General rules
Unless otherwise specified in this Standard, the purity of the used reagents shall be
analytically pure. The standard titration solution, standard solution for impurity
determination, preparations and products shall be prepared in accordance with the
provisions of GB/T 601, GB/T 602, and GB/T 603. The water used in the laboratory
shall comply with the provisions of Class-3 water specified in GB/T 6682. The solution
used in the test refers to an aqueous solution when it is not specified which solvent is
used to prepare it.
A.3 Identification test
A.3.1 Reagents and materials
A.3.1.1 Acetone.
A.3.1.2 Chloroform.
A.3.1.3 Ether.
A.3.1.4 Hydrochloric acid.
A.3.1.5 Sodium acetate.
A.3.1.6 Potassium bromide.
A.3.1.7 Sodium fluoride.
A.3.1.8 Potassium hydrogen sulfate.
A.3.1.9 Hypophosphorous acid.
A.3.1.10 Phenolphthalein indicator.
A.3.1.11 Acetic acid (1mol/L).
A.3.1.12 Sodium hydroxide solution (100g/L): Take 10g of sodium hydroxide; add
water to dissolve and make constant volume to 100mL.
m0 - the mass of the weighing bottle after drying, in g.
The calculation result retains 2 significant digits.
Take the arithmetic mean of the parallel determination results as the determination
result; and the absolute difference between the two parallel determination results shall
be no more than 1.0%.
A.6 Related substances
A.6.1 Principle of the method
After the specimen is dissolved in the mobile phase; it is detected by a liquid
chromatograph and quantified by the peak area comparison method.
A.6.2 Reagents and materials
A.6.2.1 Chloramine T.
A.6.2.2 Methanol.
A.6.2.3 Disodium hydrogen phosphate.
A.6.2.4 Phosphoric acid.
A.6.2.5 Hydrochloric acid.
A.6.2.6 Disodium hydrogen phosphate solution: 0.028mol/L. Take 10.03g of disodium
hydrogen phosphate dodecahydrate; add water to dissolve and make constant volume
to 1000mL.
A.6.2.7 Hydrochloric acid solution: 0.05mol/L. Take 4.5mL of hydrochloric acid; add
water to 1000mL; and mix well.
A.6.2.8 Chloramine T solution: 0.1%. Take 0.1g of chloramine T; add water to dissolve
and make constant volume to 100mL.
A.6.3 Apparatus
A.6.3.1 Balance: Sensitivity 0.0001g.
A.6.3.2 High performance liquid chromatograph: equipped with ultraviolet detector.
A.6.4 Analytical procedures
A.6.4.1 Specimen solution: Take 10mg of specimen into a 10mL volumetric flask;
dissolve it with mobile phase and make constant volume to the mark; and mix well.
A.6.4.2 Control solution: Accurately pipette 1mL of specimen solution in a 100mL