GB 1886.345-2021 English PDF

Delivery: 2 working-hours manually (Sales@ChineseStandard.net)
Need delivered in 3-second? USA-Site: GB 1886.345-2021
Get Quotation: Click GB 1886.345-2021 (Self-service in 1-minute)
Historical versions (Master-website): GB 1886.345-2021
Preview True-PDF (Reload/Scroll-down if blank)

GB 1886.345-2021: National food safety standard - Food additives - Mulberry red
GB 1886.345-2021
GB
NATIONAL STANDARD OF THE
PEOPLE?€?S REPUBLIC OF CHINA
National Food Safety Standard -
Food Additives - Mulberry Red
??????????????? ?????????
ISSUED ON: FEBRUARY 22, 2021
IMPLEMENTED ON: AUGUST 22, 2021
Issued by: National Health Commission of the People?€?s Republic of China;
State Administration for Market Regulation.
Table of Contents
1 Scope ... 3??
2 Molecular Formula and Structural Formula ... 3??
3 Technical Requirements ... 3??
Appendix A Inspection Method ... 5??
National Food Safety Standard -
Food Additives - Mulberry Red
1 Scope
This Standard is applicable to the food additive mulberry red, which is obtained through
extraction and refining with mulberry (Fructus Mori) fruit and its pomace as the raw
materials, and with an aqueous solution containing citric acid and edible ethanol.
2 Molecular Formula and Structural Formula
2.1 Molecular Formula
Cyanidin-3-glucoside: [C21H21O12]+ X?€?
Cyanidin-3-rutinoside: [C27H31O15]+ X?€?
X?€? is the counter ion.
2.2 Structural Formula
Cyanidin-3-glucoside: R1 = glucosyl
Cyanidin-3-rutinoside: R1 = rutinosyl
3 Technical Requirements
3.1 Sensory Requirements
The sensory requirements shall comply with the requirements of Table 1.
Appendix A
Inspection Method
WARNING: some reagents used in the inspection method of this Standard are
toxic or corrosive. During operation, appropriate safety and protective measures
shall be taken.
A.1 General Rules
When other requirements are not specified, the reagents and water used in this
Standard refer to analytically pure reagents and Grade-3 water specified in GB/T 6682.
When other requirements are not specified, the standard solutions, preparations and
products used in tests shall be prepared in accordance with the stipulations of GB/T
601, GB/T 602 and GB/T 603. When it is not specified which solvent is used for
preparation, the solutions used in tests refer to aqueous solutions.
A.2 Identification Test
A.2.1 Reagents and materials
A.2.1.1 Trifluoroacetic acid: chromatographically pure.
A.2.1.2 Acetonitrile: chromatographically pure.
A.2.1.3 Sodium hydroxide solution: 2 mol/L.
A.2.1.4 Hydrochloric acid solution: 2 mol/L.
A.2.1.5 Disodium hydrogen phosphate solution: 0.2 mol/L. Accurately weigh-take
71.64 g of disodium hydrogen phosphate (Na2HPO4 ??? 12H2O); use water to dissolve it
and dilute to a constant volume of 1,000 mL.
A.2.1.6 Citric acid solution: 0.1 mol/L. Accurately weigh-take 21.01 g of citric acid
(C6H8O7 ??? H2O); use water to dissolve it and dilute to a constant volume of 1,000 mL.
A.2.1.7 Citric acid - disodium hydrogen phosphate buffer solution: pH 3.0. Measure-
take 41 mL of 0.2 mol/L disodium hydrogen phosphate solution and 159 mL of 0.1
mol/L citric acid solution; mix them up.
A.2.1.8 Trifluoroacetic acid solution: 0.1% (volume fraction).
A.2.1.9 Extraction solvent: trifluoroacetic acid solution + acetonitrile (75 + 25).
A.2.1.10 Cyanidin-3-glucoside (kuromanin chloride) reference substance: CAS: 7084-
24-4, purity ??? 98%.
volume of 100 mL; filter them through a 0.45 ??m filter membrane.
A.2.3.3.3 Reference chromatographic conditions
A.2.3.3.3.1 Chromatographic column: C18 reversed phase column (???3 mm ??? 150 mm,
3.5 ??m), or other equivalent chromatographic columns.
A.2.3.3.3.2 Mobile phase A: trifluoroacetic acid solution (A.2.1.8).
A.2.3.3.3.3 Mobile phase B: acetonitrile (A.2.1.2).
A.2.3.3.3.4 Flow rate: 0.4 mL/min.
A.2.3.3.3.5 Detection wavelength: 520 nm.
A.2.3.3.3.6 Injection volume: 2 ??L.
A.2.3.3.3.7 Column temperature: 35 ??C.
A.2.3.3.3.8 Isocratic elution conditions: mobile phase A + mobile phase B = 89 + 11.
A.2.4 Analytical procedures
Under the reference chromatographic conditions of A.2.3.3.3, respectively inject the
reference solution and specimen solution of cyanidin-3-glucoside (kuromanin chloride)
and cyanidin-3-rutinoside.
A.2.5 Result determination
The chromatogram of the specimen solution shall manifest two obvious main peaks,
and the retention time of the two main peaks shall be consistent with the retention time
of the reference substance of cyanidin-3-glucoside (kuromanin chloride) and cyanidin-
3-rutinoside.
A.3 Determination of Color Value
A.3.1 Reagent and material
Citric acid - disodium hydrogen phosphate buffer solution: pH 3.0. Same as A.2.1.7.
A.3.2 Instruments and equipment
A.3.2.1 UV spectrophotometer.
A.3.2.2 Cuvette: 1 cm.
A.3.3 Analytical procedures
Inject the specimen solution (A.2.3.2) into a 1 cm cuvette. Take the citric acid - disodium
hydrogen phosphate buffer solution (A.2.1.7) as the blank; use the spectrophotometer
the distillation method in GB 5009.34, expressed in (g/kg);
1,000---the unit conversion factor.
---the color value of the specimen being tested.
The arithmetic mean value of parallel determination results shall prevail in the test
result. The absolute difference between two independent determination results
obtained under repeatability conditions shall not exceed 10% of the arithmetic mean
value.
A.5 pH Determination
A.5.1 Instrument and equipment
pH meter.
A.5.2 Determination method
Weigh-take 1 g of specimen, accurate to 0.01 g. Completely dissolve it in distilled water
and dilute to a constant volume of 100 mL. Use the pH meter to determine its pH value.
The arithmetic mean value of parallel determination results shall prevail in the test
result. The absolute difference between two independent determination results
obtained under repeatability conditions shall not exceed 0.1 pH.
A.6 Determination of Burning Residue
A.6.1 Instrument and equipment
A.6.1.1 Crucible.
A.6.1.2 High-temperature furnace.
A.6.1.3 Desiccator.
A.6.2 Determination method
Weigh-take 3 g of specimen, accurate to 0.001 g. Place it in a crucible that has reached
a constant mass at 800 ??C ?? 25 ??C. Firstly, slowly carbonize it on an electric furnace
(at about 300 ??C), then, transfer it into a high-temperature furnace at 800 ??C ?? 25 ??C
to burn to a constant mass.
A.6.3 Result calculation
The mass fraction w1 of the burning residue shall be calculated in accordance with
Formula (A.3).