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GB 1903.42-2020: National food safety standard - Food additive - Inositol
GB 1903.42-2020
GB
NATIONAL STANDARD OF THE
PEOPLE?€?S REPUBLIC OF CHINA
National food safety standard - Food nutritive fortifier
- Inositol (cyclohexanehexol)
ISSUED ON: SEPTEMBER 11, 2020
IMPLEMENTED ON: MARCH 01, 2021
Issued by: National Health Commission of PRC;
State Administration for Market Regulation.
Table of Contents
1 Scope ... 3??
2 Molecular formula, structural formula and relative molecular mass ... 3??
3 Technical requirements ... 3??
Appendix A Testing method ... 5??
Appendix B Inositol liquid chromatogram ... 13??
National food safety standard - Food nutritive fortifier
- Inositol (cyclohexanehexol)
1 Scope
This standard applies to inositol (cyclohexanehexol), a food nutrient fortifier
produced by hydrolysis of phytate calcium and magnesium (phenanthrene).
2 Molecular formula, structural formula and relative
molecular mass
2.1 Molecular formula
C6H12O6.
2.2 Structural formula
2.3 Relative molecular mass
180.16 (according to 2018 international relative atomic mass)
3 Technical requirements
3.1 Sensory requirements
Sensory requirements shall meet the requirements of Table 1.
3.2 Physical-chemical indicators
Appendix A
Testing method
A.1 General provisions
When other requirements are not specified, the reagents and water used in this
standard refer to analytical reagents and the level 3 water as specified in GB/T
6682. The standard solutions, standard solutions for impurity determination,
preparations and products used in the test are prepared in accordance with
GB/T 601, GB/T 602, GB/T 603, when other requirements are not specified.
When the solvent used in the test is not specified, it refers to the aqueous
solution.
A.2 Identification test
A.2.1 Reagents and materials
A.2.1.1 Ethanol.
A.2.1.2 Nitric acid.
A.2.1.3 Strontium acetate solution: 100 mg/mL. Weigh 1 g of strontium acetate.
Use water to dissolve it. Make its volume reach to 10 mL.
A.2.1.4 Litmus test paper.
A.2.2 Instruments and equipment
A.2.2.1 Electronic balance: The sensitivity is 0.01 g.
A.2.2.2 Polarimeter.
A.2.2.3 Melting point apparatus.
A.2.2.4 Water bath: The highest display temperature is 100 ??C.
A.2.3 Identification method
A.2.3.1 Acidity and alkalinity
Weigh about 1 g of specimen, accurate to 0.01 g. Add water to dissolve it. Make
its volume reach to 50 mL, to obtain the specimen solution. Put the litmus paper
into the solution. The color of the test paper shall not change.
A.2.3.2 Optical rotation
Weigh about 1 g of specimen, accurate to 0.01 g. Add water to dissolve it. Make
A.3.1.2 Reagents and materials
A.3.1.2.1 Acetic anhydride sulfuric acid solution: Take 2 mL of 1 mol/L sulfuric
acid and slowly add it dropwise to 100 mL acetic acid and let it cool.
A.3.1.2.2 Trichloromethane.
A.3.1.3 Apparatus and equipment
A.3.1.3.1 Electronic balance: The sensitivity is 0.0001 g.
A.3.1.3.2 Water bath: The highest display temperature is 100 ??C.
A.3.1.3.3 Beaker: 250 mL.
A.3.1.3.4 Watch glass.
A.3.1.3.5 Separating funnel
A.3.1.3.6 Suction filter device.
A.3.1.3.7 Constant temperature drying oven.
A.3.1.4 Determination method
Weigh about 0.2 g of specimen, accurate to 0.0001 g. Put it in a 250 mL beaker.
Add 5 mL of acetic anhydride sulfuric acid solution (A.3.1.2.1). Cover with a
watch glass. Bath at 100 ??C for 20 min. Take out the ice bath to cool. Slowly
add 100 mL of water. Heat and boil for 20 min. Take out and cool it in ice bath.
Transfer the solution to a 250 mL separatory funnel. Use a little chloroform to
rinse the beaker. Combine the rinsing solution into the separatory funnel. Use
chloroform to make extraction 6 times, respectively. The amount of chloroform
consumed is 30 mL, 25 mL, 20 mL, 15 mL, 10 mL, 10 mL. Combine the
chloroform layers in another 250 mL separatory funnel. Add 10 mL of water.
Shake it and then let it be standing. Take the chloroform layer to filter it by
absorbent cotton and collect it. Then add 10 mL of chloroform to the separatory
funnel. Shake and let it be standing. Take the chloroform layer to filter it by the
original filter and absorbent cotton to filter it. Combine the chloroform layer.
Place it in a weighed conical flask. Evaporate off the chloroform. Dry it at 105 ??C
to constant weight. The weight of the residue obtained is multiplied by 0.4167
to obtain the inositol content of the specimen. The residue can be used in
A.2.3.5 identification test.
A.3.1.5 Result calculation
The content of inositol w1 is calculated by mass fraction and calculated
according to formula (A.1):
A.3.2.4.2 Mobile phase: water.
A.3.2.4.3 Flow rate: 0.5 mL/min.
A.3.2.4.4 Injection volume: 10 ??L.
A.3.2.4.5 Column temperature: 85 ??C.
A.3.2.4.6 Differential detector temperature: 35 ??C.
A.3.2.5 Analytical steps
A.3.2.5.1 Preparation of standard solution
Accurately weigh 0.5 g of inositol standard, accurate to 0.0001 g. Place it in a
10 mL volumetric flask. Add water to dissolve and make its volume reach to the
mark.
A.3.2.5.2 Preparation of sample solution
Accurately weigh 0.5 g of specimen, accurate to 0.0001 g. Place it in a 10 mL
volumetric flask. Add water to dissolve and make its volume reach to the mark.
A.3.2.6 Determination
Under A.3.2.4 reference chromatographic conditions, measure the specimen
solution and standard solution separately. Repeat the injection twice.
Respectively record the peak area value of inositol in the corresponding
chromatogram. Calculate the content of inositol in the specimen according to
the formula in result calculation in A.3.2.7.
A.3.2.7 Result calculation
The content of inositol, w2, calculated by mass fraction, is calculated according
to formula (A.2):
Where:
A1 - The average peak area value of inositol in the specimen solution
chromatogram;
m4 - The mass of inositol in the standard solution, in grams (g);
w3 - The purity of the standard product, %;
A2 - The average peak area value of inositol in the chromatogram of the
Nessler colorimetric tube. Use 20 mL ~ 30 mL water to dissolve it. Add 10 mL
of nitric acid solution and 1 mL of silver nitrate solution. Add water to make the
volume to 50 mL. Shake slowly and let it stand for 5 min away from light. At the
same time, take 2 mL of sodium chloride standard solution and place it in a 50
mL Nessler colorimetric tube. Add 10 mL of nitric acid solution and 1 mL of silver
nitrate solution. Add water to make the volume up to 50 mL. Shake slowly and
store in the dark for 5 minutes. Place the two on a black background and
observe from the top of the colorimetric tube. The turbidity of the specimen
solution shall not be deeper than that of the control solution, then the chloride
in the specimen is less than 0.005%.
A.6 Sulfate (calculated as SO4)
A.6.1 Principle of the method
Under acidic conditions, the sulfate ions in the inositol solution reacts with the
barium chloride solution to generate barium sulfate precipitation. Compare the
turbidity with the standard solution by visual inspection.
A.6.2 Reagents and materials
A.6.2.1 Hydrochloric acid solution: 2.7 mol/L. Pipette 226 mL of hydrochloric
acid and use water to dilute it to 1000 mL.
A.6.2.2 Barium chloride solution: Weigh 12.0 g of barium chloride. Use water to
dissolve and make the volume reach to 100 mL.
A.6.2.3 Standard sulfate solution: Accurately weigh 148 mg of anhydrous
sodium sulfate. Add water to dissolve and dilute it to 100 mL. Accurately
measure 10.0 mL of the above solution and use water to dilute and make the
volume reach to 1000 mL. Each 1 mL contains 10 ??g of SO42-.
A.6.3 Analytical steps
Weigh 5 g of the specimen, accurate to 0.01 g. Place it in a 50 mL Nessler
colorimetric tube. Use 20 mL ~ 30 mL of water to dissolve it. Add 1 mL of
hydrochloric acid solution and 3 mL of barium chloride solution. Add water to
make the volume to 50 mL. Shake slowly and let it stand for 10 minutes. At the
same time, take 30 mL of sulfate standard solution and place it in a 50 mL
Nessler colorimetric tube. Add 1 mL of hydrochloric acid solution and 3 mL of
barium chloride solution. Add water to make the volume up to 50 mL. Shake
slowly and let it stand for 10 minutes. Place the two under the same black
background and observe from the top of the colorimetric tube. The turbidity of
the specimen solution shall not be deeper than the control solution, then the
sulfate in the specimen is less than 0.006%.
A.7 Burning residue