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SN/T 2034-2007: Identification of dysmicoccus grassii (Leonardi) and dysmicoccus neobrevipes beardsley
SN/T  2034‐2007 
SN
Entry-exit Inspection and Quarantine Industry
Standard of the People's Republic of China
Identification of Dysmicoccus grassii (Leonardi) and Dysmicoccus
neobrevipes Beardsley
ISSUED ON. DECEMBER 24, 2007
IMPLEMENTED ON. JULY 1, 2008
Issued by. General Administration of Quality Supervision, Inspection and Quarantine
of the People's Republic of China
Table of Contents
Foreword ... 3 
1  Scope ... 4 
2  Definitions and terms ... 4 
3  Principle ... 6 
4  Equipment and reagents... 6 
5  On-site inspection ... 6 
6  Laboratory identification ... 6 
7  Result judgment ... 9 
Annex A ... 10 
Annex B ... 11 
Annex C ... 12 
Annex D ... 13 
Foreword 
Annex A, B, C and D in this Standard are informative.
This Standard was proposed by and shall be centralized by Certification and Accreditation Administration
of the People’s Republic of China.
Responsible drafting organization of this Standard. Tianjin Entry-Exit Inspection and Quarantine Bureau
of the People’s Republic of China.
The main drafters of this Standard. Huang Guoming, Feng Jie, Niu Chunjing, QuPeng, Liu Yong, and Liu
Yueting.
This Standard is the first-time issued industry standard for exit-entry inspection and quarantine.
Identification of Dysmicoccus grassii (Leonardi) and Dysmicoccus neobrevipes
Beardsley
1  Scope 
This Standard specifies the identification of Dysmicoccus grassii (Leonardi) and Dysmicoccus
neobrevipes Beardsley for imported plants’ quarantine.
This Standard is applicable to quarantine and identification of Dysmicoccus grassii (Leonardi) and
Dysmicoccus neobrevipes Beardsley in imported quarantine goods such as fruits, seedlings, and
flowers.
2  Definitions and terms 
For the purpose of this standard, the following terms and definitions apply.
2.1
Ostioles
The structure with transverse crack like lip-shape that is inserted on the back of polypides. It normally
has 2 pairs, and few have only 1 pair. The ostioles can be divided into front ostioles and back ostioles
according to the different insertion locations. The front ostioles are on the tergum of front chest, and the
back ostioles is on the tergum of the 6th uromere.
2.2
Circulus
Circulus is located in segmental venter of the polypide. It often takes the local, cornified, and narrow
hardening frame as the boundary. Its number and size vary greatly in different coccid species. And some
species have no circulus.
2.3
Disk pore
Disk pore, also known as hole pore, is a type of secretion wax pore for coccid, including trilocular pore,
quinquelocular pore, multilocular pore, and shield disk pore.
2.4
Trilocular pore
It is a kind of disk pore, with various sizes of substantially triangular or circular hardened structures, in
which there are three microscler gland pores.
6.3.2 Identification characteristics of adult female
6.3.2.1 Dysmicoccus grassii (Leonardi)
Dysmicoccus grassii (Leonardi) adult female polypide is oval, with eight tentacles, with slender feet. The
oral-collar tubular duct is distributed on segmental venter near the propodeumcoxa. There are many
transparent pores in metapedes and tibias. There is 1 big abdominal umbilical, located between the 3rd
and 4th uromere sterna. And there are obvious side constriction and fold. Anal ring is normal. There are
inner and outer ring holes and 6 long ring hairs. It is approximately twice as long as the diameter of the
ring. The front and back ostioles are well-developed, with 2~4 hairs on the pore lobe. And the edge of
lobe have been hardened. 1There are 17 pairs of acanthopore group, 2 bore spines, five attached hairs
and a group of trilocular pores at the end-pair, located on the oval hardened piece. The hardened piece
of other acanthopore groups are significantly smaller, each with 2 bore spines (3 on head), a group of
trilocular pores and 3-5 attached hairs. There is a rectangular hardened strip in segmental venter of anal
lobe. And end-hair is as long as the ring hair. The back body only has trilocular pores that are evenly
distributed. Multilocular pore is limited at abdominal ventrite in the front and back of the circulus. It shows
in a single row on the 4th to 6th abdominal segments, and in double rows on the 7th and 8th abdominal
segments. It also shows in a single row in the front of 6th to 8th abdominal segments, and in groups after
vulva. The tubular gland is oral-collar tubular duct. And there are 2 sizes. Small oral-collar tubular duct at
the abdominal ventrite is in a horizontal row, and some ones are in the anal lobe forward to segmental
venter of the chest, with each section forming in one sub-marginal group. And minority are at the middle
of the chest. The big oral-collar tubular duct is grouped in the edge area between head and the 7th
uromere segmental venter. The trilocular pores of segmental venter are evenly distributed, but in small
quantity. Body hair is tiny on the back, but it is longer on the segmental venter. See Annex C.
Dysmicoccus grassii (Leonardi) is similar to D. orchidum. Both abdominal acanthopore groups are with
short and hard attached hairs and 2 bore spines. The differences are that - the former segmental venter
near to the propodeumcoxa has oral-collar tubular duct, but the latter has none. The former only has
transparent pores at metapedes femur and tibia; but the latter has transparent pores at metapedes coax,
trochanter, femur and tibia. The multilocular pore of former is limited to the segmental venter at the 4th
and 8th uromere; but the latter is limited to the last 2 uromeres.
Dysmicoccus grassii (Leonardi) and D. mackenziei are also similar each other. The differences are that -
there are 2 bore spines at the 2nd last pair of cerarius of Dysmicoccus grassii (Leonardi); and there are
more than 2 bore spines at the 2nd last pair of cerarius of D. mackenziei.
6.3.2.2 Dysmicoccus neobrevipes Beardsley
The Dysmicoccus neobrevipes Beardsley adult female has 8 tentacles. The eyes are hemispherical,
often with shield disk pores around the eyes. The feet are big and thick. There are many transparent
pores at metapedes femurs and tibias. Abdominal umbilical is large, with internode fold, located at the
sternum between the 3rd and 4th uromere. Anal ring is at the end of back. There are inner ring pores and
outer ring pores and 6 long ring hairs, which are approximately twice as long as the diameter of the ring.
The front and back ostioles are well-developed. And there are many short hairs and trilocular pores.
Cerarius has 17 pairs. The last pair have 2 bore spines, many attached hairs, and a group of trilocular
pores; located at the shallow hardened zone (circular, smaller than anal ring). The rest of cerarius have
2-4 thorns, few attached hairs and trilocular pores. The anal lobes are protruding. The end-hair is longer
Annex B 
(Informative) 
Distribution range and host of Dysmicoccus neobrevipes Beardsley 
B.1 Distribution range
United States (Samoa Islands, Virgin Islands, Guam, Hawaii Islands, Northern Mariana Islands), Cook
Islands, Fiji Island, Kiribati, Marshall Islands, Western Samoa, Mexico, India, Malaysia (Sabah), Pakistan,
Philippines , Singapore, Thailand, Vietnam, Italy (Sicily), Antigua and Barbuda, Bahamas Islands, Brazil,
Colombia, Costa Rica, Dominican Republic, Ecuador, Guatemala, Honduras, Haiti, Jamaica, Panama,
Peru, Puerto Rico, El Salvador, Suriname, Trinidad and Tobago Island.
B.2 Host
Tamarindus indica, Agave sisalana, Polianthes tuberosa, Mangif- era indica, Annona muricata, Annona
reticulata, Annona squamosa, Colocasia esculenta, Chrysalidocarpus Lutescens, Cocos nuci fera,
Ananas comosus, Helianthus annus, Brassica olearacea, Cucurbita maxima, Acacia farnesiana, Arachis
hypogaea, Cajanus cajan, Allium cepa, Artocarpus heterophyllus, Musa coccinea, Coffea canephora,
Guettarda speciosa, Citrus limon, Citrus sinensis, Nephelium lappaceum, Manilkara zapota,
Lycopersicon esculentum, Solanum melongena, Theobroma cacao, Tectona grandis, etc..